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KMID : 0617219960070010029
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Duksung Bulletin Phamaceutical Sciences
1996 Volume.7 No. 1 p.29 ~ p.37
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Construction of Recombinant Vaccinia Virus Vectors Containing Hepatitis B Viral Genes
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Abstract
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The hepatitis B virus (HBV) is a small, enveloped virus with a circular, double-stranded DNA genome. HBV causes active and chronic hepatitis worldwide, including Korea, and is considered to be a major factor for liver cirrhosis and hepatocellular carcinoma. In contrast to the wealth of knowledge on the gene structure and expressional regulation, immunological and pathological mechanisms for HBV-induced hepatocellular injury are not well known. In the present study, vaccinia virus, which has been demonstrated to be a useful eukaryotic expression vector, was used to clone the genes for HBV surface antigens, S and L(S+pre-S2+pre-S1). Two recombinant vaccinia virus vectors, pMJ-S and pMJ-L, which contain S and L surface antigen genes of adr-subtype HBV were constructed. The HBV-vaccinia virus recombinants obtained in this study will be used for studying the immunological aspects of HBV infection.
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KEYWORD
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